P0300 on Lexus ES: Fix & DLC Location
Factory diagnostic troubleshooting wire for fault code P0300 (P0300: Random or Multiple Cylinder Misfire Detected) specifically on the Lexus ES (2020–2026, 3.5L V6 (2GR-FKS 302HP) / 2.5L Hybrid (ES 300h 215HP) / 2.5L AWD).
1. Symptoms of P0300 on Lexus ES
When the Engine Control Module (ECM/PCM) on your Lexus ES (3.5L V6 (2GR-FKS 302HP) / 2.5L Hybrid (ES 300h 215HP) / 2.5L AWD) logs fault code P0300, drivers commonly observe:
2. Most Likely Causes on Lexus ES
Historical warranty claims and technical repair bulletins for Lexus ES point to the following failure distribution:
3. Step-by-Step DIY Repair Guide for Lexus ES
Plug a handheld OBD-II scanner into the 16-pin port under the dashboard. Check if specific cylinder codes like P0301 (Cylinder 1) or P0304 (Cylinder 4) accompany P0300. on the Lexus ES equipped with 3.5L V6 (2GR-FKS 302HP) / 2.5L Hybrid (ES 300h 215HP) / 2.5L AWD.
Remove the ignition coils, use a spark plug socket with ratchet to unthread spark plugs. Inspect electrode tips for carbon buildup, oil fouling, or burnt gaps. Replace with OEM iridium plugs gapped to spec. on the Lexus ES equipped with 3.5L V6 (2GR-FKS 302HP) / 2.5L Hybrid (ES 300h 215HP) / 2.5L AWD.
Perform an ignition swap test: Swap coil from a suspected misfiring cylinder to an adjacent cylinder. Clear codes and re-scan. If the misfire moves, replace the faulty coil. on the Lexus ES equipped with 3.5L V6 (2GR-FKS 302HP) / 2.5L Hybrid (ES 300h 215HP) / 2.5L AWD.
Inspect PCV hoses, intake boot, and vacuum lines for cracking or hissing sounds while engine idles. Smoke test the intake if necessary. on the Lexus ES equipped with 3.5L V6 (2GR-FKS 302HP) / 2.5L Hybrid (ES 300h 215HP) / 2.5L AWD.
Add a bottle of premium fuel injector cleaner (e.g. Chevron Techron). Clear the OBD-II codes with your scanner and take the car on a 15-minute drive cycle to confirm readiness monitors reset. on the Lexus ES equipped with 3.5L V6 (2GR-FKS 302HP) / 2.5L Hybrid (ES 300h 215HP) / 2.5L AWD.
Other Common Fault Codes on Lexus ES
Frequently diagnosed powertrain, emissions, and sensor trouble codes logged on the Lexus ES:
4. Lexus ES P0300 FAQs
What causes code P0300 to trigger on a Lexus ES?
On the Lexus ES (3.5L V6 (2GR-FKS 302HP) / 2.5L Hybrid (ES 300h 215HP) / 2.5L AWD), P0300 is most frequently caused by Worn or fouled spark plugs and damaged spark plug wires or Failing ignition coils or coil packs.
Where is the OBD-II port located on my Lexus ES?
On the Lexus ES, the 16-pin DLC diagnostic connector is located Directly under driver dash, left side of steering column. Plug in any standard OBD-II scanner with the ignition in the ON position to read freeze-frame parameters.
Can I safely drive my Lexus ES with code P0300?
No. Driving with a flashing check engine light causes irreversible catalytic converter damage. Given that P0300 carries a Critical severity rating, we advise avoiding heavy towing or aggressive throttle inputs until sensor readings are verified.