MazicBay Diagnostics
MAZICBAYDiagnostics
FTC Reader Disclosure: When you purchase or register through links on our site, we may earn an affiliate commission at no extra cost to you. Read our Editorial & Advertising Policy.
P0316LexusLexus LX HubAll Lexus P0316High Severity

P0316 on Lexus LX

Engine Misfire Detected on Startup (First 1000 Revolutions)

Factory diagnostic protocol and troubleshooting wire for fault code P0316 specifically for the Lexus LX (2022–2026, 3.4L Twin-Turbo V6 (V35A-FTS LX 600 409HP) / Hybrid 700h).

Verified OEM Telemetry & Field Repair Tree
Lexus LX2022–2026
Lexus LX
SUV3.4L Twin-Turbo V6 (V35A-FTS LX 600 409HP) / Hybrid 700h
Reliability
Sensor Voltage Ref
0.1V – 5.0V Ref
LX SAE Mode $01
ECU Network Bus
500 kbps CAN-FD
ISO 15765-4 Compliant
Drivability Status
Drivable (Short Trip)
High urgency level
Est. Repair Cost
$100 - $600 (DIY: $30 - $150)
Parts & Labor DIY
Check P0316 On Another Lexus Model:
Diagnose P0316 on Specific LX Model Year:

1. Symptoms of P0316 on Lexus LX

When the Engine Control Module (ECM/PCM) on your Lexus LX (3.4L Twin-Turbo V6 (V35A-FTS LX 600 409HP) / Hybrid 700h) logs fault code P0316, drivers commonly observe:

Check Engine Light on on Lexus LX (3.4L Twin-Turbo V6 (V35A-FTS LX 600 409HP) / Hybrid 700h)
Engine shakes, sputters, and runs very rough immediately after cold start on Lexus LX (3.4L Twin-Turbo V6 (V35A-FTS LX 600 409HP) / Hybrid 700h)
Engine smooths out and runs normally after warming up for 1–2 minutes on Lexus LX (3.4L Twin-Turbo V6 (V35A-FTS LX 600 409HP) / Hybrid 700h)
Accompanying misfire codes (P0300, P0301-P0308) on Lexus LX (3.4L Twin-Turbo V6 (V35A-FTS LX 600 409HP) / Hybrid 700h)
Lexus LX OBD-II Port Location
The 16-pin diagnostic connector is located Under driver steering column behind flip cap. Plug in your scan tool to verify freeze-frame sensor values.

2. Most Likely Causes on Lexus LX

Historical warranty claims and technical repair bulletins for Lexus LX point to the following failure distribution:

55%
Coolant leaking into cylinder overnight from failing head gasket or intake manifold (Lexus OEM Part)
25%
Leaking fuel injector dripping fuel into cylinder while parked (Lexus OEM Part)
12%
Worn spark plugs with excessive gap or moisture on ignition boots (Lexus OEM Part)
8%
Carbon buildup on intake valves preventing cold fuel vaporization (Lexus OEM Part)
* Source: Synthesized from NHTSA ODI field repair telemetry and verified OEM Technical Service Bulletin (TSB) repair tree frequency percentiles for Lexus LX.

3. Step-by-Step DIY Repair Guide for Lexus LX

01
Check Accompanying Cylinder Misfire Code

Check if P0316 is accompanied by a specific cylinder code (like P0303) to isolate which cylinder is misfiring on startup. on the Lexus LX equipped with 3.4L Twin-Turbo V6 (V35A-FTS LX 600 409HP) / Hybrid 700h.

Required Tool: OBD-II Scanner
02
Perform Cooling System Pressure Test

Pressurize cold cooling system to 15 PSI. Use a borescope camera through spark plug hole to check for coolant pool on piston top. on the Lexus LX equipped with 3.4L Twin-Turbo V6 (V35A-FTS LX 600 409HP) / Hybrid 700h.

Required Tool: Cooling System Pressure Tester, Borescope Camera
03
Inspect Spark Plugs and Coils

Remove spark plugs. Check for white coolant crust, fuel fouling, or cracked ceramic insulators. on the Lexus LX equipped with 3.4L Twin-Turbo V6 (V35A-FTS LX 600 409HP) / Hybrid 700h.

Required Tool: Spark Plug Socket, Ratchet
04
Perform Intake Valve Carbon Cleaning

On direct-injection engines, perform walnut shell blasting or chemical intake valve cleaning to remove cold carbon buildup. on the Lexus LX equipped with 3.4L Twin-Turbo V6 (V35A-FTS LX 600 409HP) / Hybrid 700h.

Required Tool: Intake Valve Cleaner Kit
05
Clear Trouble Codes and Test Overnight Cold Start

Clear DTCs and allow vehicle to sit overnight to confirm morning start is clean. on the Lexus LX equipped with 3.4L Twin-Turbo V6 (V35A-FTS LX 600 409HP) / Hybrid 700h.

Required Tool: OBD-II Scanner
Recommended Parts & Testing Tools

Equipment for Diagnosing Lexus LX (P0316)

Prime Fast Delivery & Verified Fitment
Direct-Fit Replacement Part

Lexus LX OEM Replacement Sensor

4.7 / 5.0 (Verified Fit)

Direct-fit replacement assembly engineered to OEM tolerances for Lexus LX.

$45 – $95 Prime
Check Fitment on Amazon
Sensor Signal Voltage Meter

AstroAI Auto-Ranging Digital Multimeter

4.6 / 5.0 (Best Seller)

Essential for testing 0.1V–5.0V sensor reference signals, ground continuity (<0.20Ω), and harness resistance.

$29.99 Prime
View on Amazon
Top Mobile OBD-II Scanner

BlueDriver Bluetooth Pro OBDII Scan Tool

4.7 / 5.0 (50,000+ Reviews)

Reads and clears enhanced codes (ABS, Airbag, Transmission, Engine) & live freeze-frame telemetry on phone.

Compare OEM & Aftermarket Replacement Sensors for Lexus LXVerified direct-fit pricing from Denso, Bosch, Motorcraft, and ACDelco on Amazon.
Find Parts on Amazon
* As an Amazon Associate, MazicBay earns from qualifying purchases. Prices & availability subject to change.

Other Common Fault Codes on Lexus LX

Frequently diagnosed powertrain, emissions, and sensor trouble codes logged on the Lexus LX:

4. Lexus LX P0316 FAQs

What causes code P0316 to trigger on a Lexus LX?

On the Lexus LX (3.4L Twin-Turbo V6 (V35A-FTS LX 600 409HP) / Hybrid 700h), P0316 is most frequently caused by Coolant leaking into cylinder overnight from failing head gasket or intake manifold or Leaking fuel injector dripping fuel into cylinder while parked.

Where is the OBD-II port located on my Lexus LX?

On the Lexus LX, the 16-pin DLC diagnostic connector is located Under driver steering column behind flip cap. Plug in any standard OBD-II scanner with the ignition in the ON position to read freeze-frame parameters.

Can I safely drive my Lexus LX with code P0316?

Caution. Engine misfires heavily for the first 30–60 seconds after starting, then smooths out. Given that P0316 carries a High severity rating, we advise avoiding heavy towing or aggressive throttle inputs until sensor readings are verified.