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P0140 on Toyota Grand Highlander

O2 Sensor Circuit No Activity (Bank 1 Sensor 2)

Factory diagnostic protocol and troubleshooting wire for fault code P0140 specifically for the Toyota Grand Highlander (2024–2026, 2.4L Turbo / 2.5L Hybrid / Hybrid MAX 362HP Turbo AWD).

Verified OEM Telemetry & Field Repair Tree
Toyota Grand Highlander2024–2026
Toyota Grand Highlander
SUV2.4L Turbo / 2.5L Hybrid / Hybrid MAX 362HP Turbo AWD
Reliability
Sensor Voltage Ref
0.1V – 5.0V Ref
Grand Highlander SAE Mode $01
ECU Network Bus
500 kbps CAN-FD
ISO 15765-4 Compliant
Drivability Status
Drivable (Short Trip)
Low urgency level
Est. Repair Cost
$100 - $300 (DIY: $35 - $100)
Parts & Labor DIY
Check P0140 On Another Toyota Model:
Diagnose P0140 on Specific Grand Highlander Model Year:

1. Symptoms of P0140 on Toyota Grand Highlander

When the Engine Control Module (ECM/PCM) on your Toyota Grand Highlander (2.4L Turbo / 2.5L Hybrid / Hybrid MAX 362HP Turbo AWD) logs fault code P0140, drivers commonly observe:

Check Engine Light on on Toyota Grand Highlander (2.4L Turbo / 2.5L Hybrid / Hybrid MAX 362HP Turbo AWD)
Downstream sensor flatlined at static 0.45V bias voltage on Toyota Grand Highlander (2.4L Turbo / 2.5L Hybrid / Hybrid MAX 362HP Turbo AWD)
Emissions readiness monitor incomplete on Toyota Grand Highlander (2.4L Turbo / 2.5L Hybrid / Hybrid MAX 362HP Turbo AWD)
Toyota Grand Highlander OBD-II Port Location
The 16-pin diagnostic connector is located Driver side lower knee panel above dead pedal. Plug in your scan tool to verify freeze-frame sensor values.

2. Most Likely Causes on Toyota Grand Highlander

Historical warranty claims and technical repair bulletins for Toyota Grand Highlander point to the following failure distribution:

55%
Completely dead downstream O2 sensor (Toyota OEM Part)
25%
Disconnected sensor harness plug under car (Toyota OEM Part)
12%
Cut or broken signal wire in vehicle harness (Toyota OEM Part)
8%
Corroded terminal pins in connector (Toyota OEM Part)
* Source: Synthesized from NHTSA ODI field repair telemetry and verified OEM Technical Service Bulletin (TSB) repair tree frequency percentiles for Toyota Grand Highlander.

3. Step-by-Step DIY Repair Guide for Toyota Grand Highlander

01
Check Sensor Connection Underbody

Inspect harness connector under vehicle floor pan. Verify plug is firmly seated and weather seal is intact. on the Toyota Grand Highlander equipped with 2.4L Turbo / 2.5L Hybrid / Hybrid MAX 362HP Turbo AWD.

Required Tool: Flashlight, Jack Stands
02
Test Sensor Heater & Signal Circuit

Check for 12V heater power and ground at connector with ignition ON. on the Toyota Grand Highlander equipped with 2.4L Turbo / 2.5L Hybrid / Hybrid MAX 362HP Turbo AWD.

Required Tool: Multimeter
03
Replace Downstream Oxygen Sensor

Remove and replace the downstream oxygen sensor with an OEM-spec part. on the Toyota Grand Highlander equipped with 2.4L Turbo / 2.5L Hybrid / Hybrid MAX 362HP Turbo AWD.

Required Tool: O2 Sensor Socket, Ratchet
04
Clear DTC Codes

Clear code and verify sensor activity returns on live telemetry. on the Toyota Grand Highlander equipped with 2.4L Turbo / 2.5L Hybrid / Hybrid MAX 362HP Turbo AWD.

Required Tool: OBD-II Scanner
Recommended Parts & Testing Tools

Equipment for Diagnosing Toyota Grand Highlander (P0140)

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Direct-Fit Replacement Part

Toyota Grand Highlander OEM Replacement Sensor

4.7 / 5.0 (Verified Fit)

Direct-fit replacement assembly engineered to OEM tolerances for Toyota Grand Highlander.

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Sensor Signal Voltage Meter

AstroAI Auto-Ranging Digital Multimeter

4.6 / 5.0 (Best Seller)

Essential for testing 0.1V–5.0V sensor reference signals, ground continuity (<0.20Ω), and harness resistance.

$29.99 Prime
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Top Mobile OBD-II Scanner

BlueDriver Bluetooth Pro OBDII Scan Tool

4.7 / 5.0 (50,000+ Reviews)

Reads and clears enhanced codes (ABS, Airbag, Transmission, Engine) & live freeze-frame telemetry on phone.

Compare OEM & Aftermarket Replacement Sensors for Toyota Grand HighlanderVerified direct-fit pricing from Denso, Bosch, Motorcraft, and ACDelco on Amazon.
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Other Common Fault Codes on Toyota Grand Highlander

Frequently diagnosed powertrain, emissions, and sensor trouble codes logged on the Toyota Grand Highlander:

4. Toyota Grand Highlander P0140 FAQs

What causes code P0140 to trigger on a Toyota Grand Highlander?

On the Toyota Grand Highlander (2.4L Turbo / 2.5L Hybrid / Hybrid MAX 362HP Turbo AWD), P0140 is most frequently caused by Completely dead downstream O2 sensor or Disconnected sensor harness plug under car.

Where is the OBD-II port located on my Toyota Grand Highlander?

On the Toyota Grand Highlander, the 16-pin DLC diagnostic connector is located Driver side lower knee panel above dead pedal. Plug in any standard OBD-II scanner with the ignition in the ON position to read freeze-frame parameters.

Can I safely drive my Toyota Grand Highlander with code P0140?

Yes, safe for normal driving without risk of breakdown. Given that P0140 carries a Low severity rating, we advise avoiding heavy towing or aggressive throttle inputs until sensor readings are verified.